蒜氨酸与大蒜素抑制肿瘤坏死因子α诱导的炎症反应对比

Comparative Study of Alliin and Allicin Inhibit Tumor Necrosis Factor-α Induced Inflammation

目的比较蒜氨酸与大蒜素对肿瘤坏死因子α诱导的炎症反应的抑制作用,并进一步探讨蒜氨酸能否取代大蒜素的抗动脉粥样硬化作用。方法将细胞分成10组:对照组、肿瘤坏死因子α组(1μg/L)、蒜氨酸组、大蒜素组、肿瘤坏死因子α(1μg/L)+蒜氨酸(5 mg/L、10 mg/L、30 mg/L)组、肿瘤坏死因子α(1μg/L)+大蒜素(5mg/L、10 mg/L、30 mg/L)组,分别培养16 h。提取各组细胞mRNA,用实时定量PCR测定细胞间黏附分子1的表达水平;用虎红染色法检测人单核细胞与人脐静脉内皮细胞的黏附性;用划线法和MTT法分别检测大蒜素和蒜氨酸对平滑肌细胞迁移和增殖作用的影响。结果蒜氨酸和大蒜素预处理都能有效抑制肿瘤坏死因子α诱导的细胞间黏附分子1表达量的增高,并减弱由其导致的单核细胞对人脐静脉内皮细胞的黏附性增加,同时能抑制由肿瘤坏死因子α诱导的平滑肌细胞迁移和增殖作用。结论蒜氨酸与大蒜素对肿瘤坏死因子α介导的炎症反应及动脉粥样硬化均有一定的抑制作用,并且效果相近,因此蒜氨酸一定程度上可以取代大蒜素。

Aim To compare alliin and allicin＇ s effect in inhibiting tumor necrosis factor-α （TNF-α） induced inflammation, and to investigate whether alliin can substitute allicin in resisting atherosclerosis. Methods Human umbilical vein endothelial cell （HUVEC） were divided into ten groups and treated for 16 hours as follows：Control（ treated without any drugs）, TNF-α（1 μg/L）, alliin （10 mg/L）, allicin （10 mg./L）, TNF-α（1 μg/L） and alliin （5 mg/L,10 mg/L,30 mg/L） ,TNF-α （1 μg/L） and allicin（5 mg/L, 10 mg/L,30 mg/L）. The mRNA expression level of intercellu- lar cell adhesion molecule-1 （ICAM-1） was determined by real-time PCR. THP-1 adhesion to HUVEC was measured by rose Bengal staining. Migration function and MTT text were used to demonstrate the effects of alliin and aHicin on the smooth muscle cell＇ s migration and proliferation. Results TNF-α treatment significantly increased the mRNA expression of ICAM-1 in HUVEC, while alliin and allicin pretreatment all blocked the TNF-α induced mRNA expression of ICAM-1, and also effectively blocked THP-1 adhesions. Further more, alliin and allicin inhibited TNF-α induced con- tractile fiber cell＇ s migration and proliferation functions. However, allicin was a bit more effective than alliin. Conclusion Both alliin and allicin can inhibit TNF-α induced inflammation and atherosclerosis, and alliin may substitute allicin in resisting atherosclerosis to a certain extent.