结缔组织生长因子受体结合区域的预测及筛选

Prediction and Screening of CTGF Secondary Structure and Receptor-binding Domain

目的分析结缔组织生长因子(connective tissue growth factor,CTGF)二级结构,进行其受体结合区域的预测及筛选。方法利用生物信息学手段,分析CTGF二级结构、亲水性以及其它部分物理性质,预测和筛选出CTGF与受体的可能结合区域,为以后人工合成小分子多肽的CTGF拮抗剂做出初步筛选。结果通过对CTGF二级结构、相应同源蛋白对比、以及亲水性和抗原性分析结果显示,CTGF与其受体结合的位点可能位于96～102、104～112、257～272区段,它们的氨基酸序列分别是TAKDGAP、IFGGTVYRS和IRTPKISKPIKFELSG。结论初步筛选出CTGF受体结合位点,为根据其3个氨基酸序列合成CTGF拮抗剂提供了工作基础。

Objective To analyze the secondary structure of connective tissue growth factor（CTGF） for the prediction and screening of candidate receptor-binding domain of CTGF.Methods Bioinformatics method was employed to predict and screen CTGF receptor-binding domain based on the analysis of secondary structure of CTGF,and its hydrophilicity and physical property.Results The results showed that the candidate receptor-binding domains locate in 96-102,104-112,257-272 segments,the corresponding amino acid sequences are TAKDGAP,IFGGTVYRS and IRTPKISKPIKFELSG,respectively.Conclusion There were 3 candidate receptor-binding domains of CTGF,which might be the targets for newly antagonistic micromolecule polypeptide of CTGF.