人增生性瘢痕成纤维细胞的改良培养和鉴定

Refined culture and identification of human hypertrophic scar fibroblasts

目的:探讨并建立一种简便易行、培养周期短的成纤维细胞改良培养方法。方法:采用组织块贴壁结合胰蛋白酶消化法体外分离培养人增生性瘢痕成纤维细胞。倒置相差显微镜下观察培养的成纤维细胞形态,进行细胞鉴定,作生长曲线观察细胞增殖能力。结果:采用组织块贴壁结合胰蛋白酶消化法体外分离人增生性瘢痕成纤维细胞,细胞培养周期短。分离培养的成纤维细胞波形蛋白呈阳性表达。传代培养的成纤维细胞增殖能力强。结论:采用组织块贴壁结合胰蛋白酶消化法体外分离培养法可以较快、较好地获得稳定培养的增生性瘢痕成纤维细胞,为其形成机制及防治研究提供实验基础。

Objective To investigate an easy and short cycle method of culturing hypertrophic scar fibroblasts in vitro. Methods Isolation and culture of hypertrophic scar fibroblasts were administered by the method which combined tissue explants adherent and enzyme digestion. The morphological changes of fibroblasts were observed under inverted microscope, then the fibroblasts were identified with immunocytoehemistry, and the proliferation assay was performed with growth curve. Results The culture cycle of the method which combined tissue explants adherent and enzyme digestion was short. The expression of vimentin protein was positive in fibroblasts cultured. The subcultured fibroblasts possessed a strong reproductive ability. Conclusion Stable fibroblasts were cultured more easily and quickly with the method combining the tissue explants adherent and trypsin digestion.