西洛他唑对强噪声所致豚鼠耳蜗血流及听阈偏移的影响

Effects of cilostazol on cochlear blood flow and hearing threshold shift in guinea pigs after noise exposure

目的:探讨在强噪声条件下,磷酸二酯-3A(phosphodiasterase-3A,PDE3A)特异性抑制剂西洛他唑(cilostazol)对豚鼠耳蜗血流及听阈偏移的影响。方法:将40只雄性豚鼠随机分为4组(每组10只):对照组、无噪声给药组、强噪声不给药组,强噪声给药组。强噪声组给予4 KHz的倍频窄带噪声(105 dB SPL)处理13 h。处理后立即腹腔注射西洛他唑(10 mg/kg),分别测定处理后各组动物的耳蜗血流速度及ABR阈值。结果:与对照组相比较,强噪声不给药组的血流速度在处理后60、90、120 min时均显著性下降(P<0.01);与强噪声不给药组比较,强噪声给药组的血流速度在处理后30、60、90、120 min时均显著性增高(P<0.05)。与对照组相比较,强噪声不给药组在处理后ABR阈值显著性高于对照组(P<0.05);与强噪声组不给药组相比较,强噪声组给药组ABR阈值要显著性降低(P<0.01)。

Objective To explore the effects of cilostazol, a PDE3A inhibitor, on cochlear blood flow and hearing threshold shift in guinea pigs after being exposed to intense noise. Methods Forty male guinea pigs were randomly divided into four groups： Group 1 was the control. Group 2 was only treated with cilostazol with a concentration of 10 mg/kg by intraperitoneal injection. Group 3 was exposed to 105 dB SPL with 4 kHz noise for 13 h. Group 4 was exposed to 105 dB SPL with 4 kHz noise for 13 h and then treated with 10 mg/kg cilostazol. After treatment, the cochlear blood flows and ABR thresholds of all groups were measured, respectively. Results Comparing to the control, the cochlear blood flow of Group 3 significantly decreased at 60, 90 and 120min, after noise exposure （P 〈 0.05）. Comparing to Group 3, the cochlear blood flow of Group 4 significantly increased at 30, 60, 90 and 120 min, after drug treatment （P 〈 0.05）. Comparing to the control, the ABR threshold of Group 3 significantly decreased after noise exposure （P 〈 0.05）. Comparing to Gourp 3, the cochlear blood flow of Group 4 significantly increased after drug treatment （P 〈 0.01 ）. Conclusion Cilostazol can effectively improve the cochlear blood flow and recover the ABR threshold shift of the guinea pigs after being exposed to intense noise.