GPC3与AFP在肝硬化异型增生结节及肝细胞癌中的表达及意义

Expression of GPC3 and AFP in Dysplastic Nodules of Cirrhosis and Hepatocellular Carcinoma

目的观察GPC3、AFP在肝硬化高度异型增生结节及肝细胞癌中的表达,探讨其在肝细胞癌诊断及鉴别诊断中的作用及意义.方法应用免疫组织化学Envision法检测GPC3、AFP在肝硬化高度异型增生结节、肝细胞癌及正常肝组织中的表达.结果 GPC3在进展期HCC中的表达阳性率为86.36%(38/44),在高分化HCC中的表达阳性率为68.18%(15/22),在肝硬化伴高度异型增生结节中的表达阳性率为45%(9/20),在正常肝组织中无阳性表达(0/20).AFP在进展期HCC中的表达阳性率为47.72%(21/44),在高分化HCC中的表达阳性率为27.27%(6/22),在肝硬化伴高度异型增生结节中的表达阳性率为15%(3/20),在正常肝组织中无阳性表达(0/20).结论 GPC3是一个较敏感及特异的标记物,可以和AFP联合应用协助诊断肝细胞癌.

Objective To study the expression of GPC3 and AFP in high dysplastic nodules of cirrhosis and hepatocellular carcinoma, and discuss its significance in the hepatocellular carcinoma and high dysplastic nodules of cirrhosis. Method By using immunohistochemical method, we examined the expression of CPC3 and AFP in high dysplastic nodules of cirrhosis, hepatocellular carcinoma and normal liver tissues. Results The positive expression rate of GPC3 was 86.36% （38/44） in advanced HCC; 68.18% （15/22） in well - differentiated HCC; 45% （9/20） in high dysplastic nodules of cirrhosis; no expression in normal liver tissue （0/20）. The positive expression rate of AFP was 47.72% （21/44） in advanced HCC; 27.27% （6/22） in well-differentiated HCC; 15% （3/20） in high dysplastic nodules of cirrhosis; no expression in normal liver tissue （0/20）. Conclusion GPC3 is a relatively sensitive and specific marker, and can be used with AFP in pathologic diagnosis of HCC.