2型糖尿病患者抵抗素基因-420C/G位点多态性与高血压相关性研究

Relationship between 420C/G Polymorphism of Resistin and Hypertension in T2DM Patients

目的探讨2型糖尿病患者抵抗素基因-420C/G位点多态性与高血压的相关性.方法选取正常对照组72例,2型糖尿病组40例,2型糖尿病合并高血压组患者40例,利用PCR—限制性内切酶消化的方法检测抵抗素基因-420C/G位点多态性,比较和分析各组间基因型频率和等位基因频率及其与高血压危险因素的相关性.结果 (1)酶切后可见3种带型:CC型,CG型,GG型;(2)2型糖尿病合并高血压组C/G等位基因频率及基因型分布与正常对照组比较差异有统计学意义(P<0.05);(3)2型糖尿病组C/G等位基因频率及基因型分布与正常对照组比较差异无统计学意义(P>0.05);(4)2型糖尿病合并高血压组抵抗素基因SNP-420C>G多态性与高血压危险因素相关指标的比较结果:舒张压、年龄水平在含G等位基因组明显高于C等位基因组(P<0.05).结论 2型糖尿病患者抵抗素基因-420C/G位点多态性与高血压具有一定相关性.

Objective To explore the association between 420 C/G polymorphism of resistin and hypertension in T2DM patients. Methods 72 healthy subjects, 40 T2DM patients and 40 patients with T2DM and hypertension were selected in normal control group, T2DM group and T2DM and hypertension group, respectively. The single nucleotide polymorphism （SNP） of resistin in three groups was demonstrated by PCR and restriction endonuclease digestion. The genotype frequency and allele frequency were compared among three groups. Results （1） There were three genotypes at the site of -420 of resistin gene：CC, CG and GG. （2） There were significant differences in the CC/ （CG＋GG） genotype and the C/G allelic frequencies between T2DM patients with hypertention and healthy subjects （P = 0.0196）. （3） There was no significant difference in genotype frequencies or alle[ frequencies between healthy subjects and T2DM patients （P = 0.2411 ）. （4） Resistin gene SNPs-420C 〉 G genotype was compared with the risk factors of hypertention in T2DM with hypertention group： diastolic blood pressure, age were significiantly higher in the CG＋GG subjects than those in CC subjects （P 〈 0.05）. Conclusion There is a correlation between resistin gene -420C/G polymorphism and hypertention in type 2 diabetes.