食蟹猴上肢取食执行时间的随龄变化及其与^(99m)Tc-TRODAT-1摄取率的相关关系研究

Age-related changes of upper limb performance time and its association with the uptake ratio of ^(99m)Tc-TRODAT-1 in normal cynomolgus monkeys

目的研究食蟹猴随年龄增长伴随的用上肢取食执行时间的变化及其与纹状体99mTc-TRODAT-1〔锝99-2β-[N,N'-双(2-巯乙基)乙撑二胺基]甲基,3β-(4-氯苯基)托烷〕摄取率之间的相关关系。方法选取4、10和15岁3个年龄组的健康食蟹猴共30只,利用改良的运动活动平板定量测定上肢取食执行时间,各年龄组分别选取4只动物用多巴胺转运体(dopamine transporter,DAT)配体99mTc-TRODAT-1结合的单光子发射体层摄影术(single-photon emission computed tomography,SPECT)显像观察脑内纹状体多巴胺转运体放射性摄取率(99mTc-TRODAT-1摄取率)的变化。结果随着年龄的增长,食蟹猴用上肢取食执行时间逐渐延长,纹状体99mTc-TRODAT-1放射性摄取率呈逐渐减低的趋势,直线回归分析结果显示食蟹猴上肢取食执行时间与年龄呈统计学相关关系,且与纹状体99mTc-TRODAT-1放射性摄取率亦呈统计学的相关关系。进一步的独立于年龄的部分相关分析表明年龄增长驱动2者的相关。结论正常食蟹猴随年龄增长脑内99mTc-TRODAT-1摄取率的减少是造成食蟹猴上肢取食执行时间延长的关键因素之一。

Objective The present study investigates age-related changes of upper limb performance time and its correlation with the aging-associated alterations of uptake ratio of 99mTc-TRODAT-1. Methods A total of 30 cynomolgus monkeys ranging from 3.5 to 15.5 years old were studied.The upper limb performance was quantitatively assessed using a modified movement assessment panel（MAP）,and the function of the dopaminergic system represented by striatal dopamine transporter（DAT） uptake ratio was semi-quantatively detected using 99mTc-TRODAT-1 single-photon emission computed tomography（SPECT） imaging. Results A significant slowness of upper limb performance time was associated with aging and significantly correlated with age-related decrease in 99mTc-TRODAT-1 uptake ratio.A further partial correlational analysis independent of age indicates that aging is inclined to drive the relationship between striatal DAT levels and upper limb performance time. Conclusion Our results suggest that aging-associated dysfunction of dopaminergic function is at least an important factor underlying the age-related slowness of upper limb performance time in normal cynomolgus monkeys.