PTTG、bFGF及PCNA在垂体腺瘤中的表达及其与侵袭性的关系

Expression of pituitary tumor transforming gene,basic fibroblast growth factor and proliferating cell nuclear antigen in human pituitary adenomas:correlation with invasiveness

目的通过比较垂体腺瘤侵袭组与非侵袭组之间垂体瘤转化基因(pituitary tumor transforming gene,PTTG)、细胞增生核抗原(proliferating cell nuclear antigen,PCNA)、碱性成纤维细胞生长因子(basic fibroblast growth factor,bFGF)mRNA表达水平的差异来探讨它们和垂体腺瘤侵袭性之间的关系,为临床垂体腺瘤侵袭性判定及预后评估提供可能的生物学指标。方法选取首都医科大学附属天坛医院神经外科临床资料完整、行手术治疗并经病理证实为垂体腺瘤的肿瘤标本65例,分为侵袭组和非侵袭组,用实时定量反转录PCR(RT-PCR)方法检测肿瘤组织中PTTG、PCNA、bFGF 3者mRNA表达水平,3者间两两比较表达水平的差异。结果侵袭组与非侵袭组之间PTTG和PCNA的表达差异有统计学意义(P<0.05),而bFGF的表达在2组之间差异无统计学意义(P>0.05);PTTG和bFGF的表达呈正相关(r=0.26,P=0.038)。结论 PTTG和PCNA和垂体腺瘤的侵袭性生长有密切关系,可能作为垂体腺瘤侵袭性生长的重要标志物;PTTG和bFGF的互相诱导表达可能促进了人垂体腺瘤的形成、发展,并参与侵袭的发生。

Objective To evaluate the relationship between mRNA expression of pituitary tumor transforming gene（PTTG）,proliferating cell nuclear antigen（PCNA）,basic fibroblast growth factor（bFGF） and the aggressiveness of pituitary adenomas in 65 patients. Methods Tumor specimens from 65 patients with pathologically confirmed pituitary adenoma were selected for this study.The tumor specimens included 40 invasive adenomas and 25 non-invasive adenomas grouped according to MRI scan.The mRNA expression of PTTG,PCNA and bFGF in the pituitary adenoma tissue was assayed by reverse transcription polymerase chain reaction（RT-PCR）,and a correlation analysis was performed. Results PCNA and PTTG expression was significantly higher in invasive group than in noninvasive group（P0.05）,whereas,there was no significant difference in bFGF expression between those two groups（P0.05）.The correlation analysis revealed that there was a positive correlation between bFGF and PTTG（r=0.26,P=0.038）. Conclusion PTTG and PCNA are both correlated with the invasiveness of pituitary adenoma and may be a valuable biological predicting marker for invasion;PTTG expression parallels that of bFGF and they possibly have synergic effect to promote the invasiveness of pituitary adenomas.