脑动脉狭窄患者血清中炎性因子干扰素-γ、白细胞介素-6和肿瘤坏死因子-α的水平分析

Serum levels of interferon-γ,interleukin-6 and tumor necrosis factor-α in patients with cerebral arterial stenosis

目的:研究不同类型脑动脉狭窄患者血清中干扰素(interferon,IFN)-γ、白细胞介素(interleukin,IL)-6和肿瘤坏死因子(tumor necrosis factor,TNF)-α的水平,分析这几种炎性因子在不同类型脑动脉狭窄患者血清中的变化规律。方法:按脑动脉狭窄部位分为颅内狭窄组(26例),颅外狭窄组(30例),颅内、外混合狭窄组(27例)及单纯大脑中动脉(middle cerebral artery,MCA)狭窄组(25例),另外包括对照人群33例。通过酶联免疫吸附试验(en-zyme linked immunosorbent assay,ELISA)检测血清中IFN-γ、IL-6和TNF-α的含量并采用SPSS 11.5统计分析软件进行分析比较。结果:对血清中3种因子的检测发现,颅内狭窄组、颅外狭窄组、混合狭窄组和MCA狭窄组血清中的IFN-γ和IL-6均显著高于对照组(P=0.000),但在各狭窄组间IFN-γ差异无统计学意义,而颅内狭窄组的IL-6含量低于其他狭窄组(P<0.05)。对TNF-α的检测发现,只有颅内狭窄组和MCA狭窄组显著高于对照组(P<0.05),而颅外狭窄组和混合狭窄组与对照组相比差异无统计学意义(P>0.05)。结论:上述实验结果提示IFN-γ、IL-6和TNF-α可能参与某些类型脑动脉狭窄的形成过程。

Objective：To investigate the relationship between interferon-γ（IFN-γ）,interleukin-6（IL-6）,tumor necrosis factor-α（TNF-α） and cerebral arterial stenosis and to compare serum levels of these inflammatory cytokines in different cerebral arteries stenosis groups.Methods： According to the stenotic site,patients with cerebral arteries stenosis were divided into intracranial stenosis group（n=26）,extracranial stenosis group（n=30）,intracranial and extracranial stenosis group（n=27）,and group of stenosis in middle cerebral artery（n=25）.And there were 33 samples in the control group.Blood serum IFN-γ,IL-6 and TNF-α levels were analyzed using enzyme linked immunosorbent assays（ELISA）.Results： Serum IFN-γ and IL-6 levels were significantly increased in all the stenosis groups（P=0.000） as compared with the control group.But serum IFN-γ level had no significant difference between the two optional stenosis groups.And IL-6 level in intracranial stenosis group was lower than those in the other stenosis groups（P0.05）.In addition,we found that TNF-α of intracranial stenosis group（P=0.001） and MCA stenosis group（P=0.015） significantly increased as compared with the control group and the other two stenosis groups.Conclusion： The results suggest that IFN-γ,IL-6 and TNF-α are possible to participate in the formation of certain cerebral arterial stenosis.