复方光明胶囊对实验性大鼠视网膜光损伤的作用

Effect of Compound Light Capsule on Rats' Retinal Light Injury Induced by Operation Microscope,an Electron Microscope Observation

目的研究视网膜光损伤动物模型,观察复方光明胶囊对手术显微镜光损伤大鼠视网膜作用的超微结构.方法SD大鼠30只(60只眼),随机分为高、中、低剂量组,模型组和正常组,每组6只动物(12只眼).造模前7 d给药,连续灌胃2周.除正常组以外各组实验用大鼠距角膜(150±3)mm照射,使大鼠眼在接受手术显微镜水平位时照度为(22 000±1 000)Lux,60 min的持续光照射.光照后继续给药1周,观察各组视网膜电镜的变化差异.结果正常大鼠视网膜组织结构层次清楚,内、外节视杆排列整齐、规则.内、外核层排列紧密,染色均匀;光照后各组均可见外核层变薄而稀疏;光感受器视杆外节排列紊乱,膜盘间隙部分断裂;内节线粒体肿胀;外核层细胞核染色质固缩,分布不均匀.药物剂量高、中、低组较模型组有改善.结论手术显微镜视网膜光损伤动物模型造模成功,复方光明胶囊各剂量组对视网膜光损伤后有一定的保护作用.

Objective To establish the rat model of retinal light injury,and observe the effect of Compound Light Capsule on the rat retinal light injury under electron microscope.Methods 30 SD rats（60 eyes）were divided into high dose group,middle dose group,low dose group,sham group,and normal group in random,6 SD rats（12 eyes）in each group.Rats were intragastrically administered with Compound Light Capsule 7 days before modeling,and the administration lasted for 2 weeks.Experimental rats in each group were exposured to the light away from the corner（150±3） mm except the normal group.The illumination of eyes in the horizontal position of operation microscope was（22 000±1 000） lux,and continue light exposure for 30 minutes.The administration for another 1 week after exposure to the light.The retinal morphology changes of each group were observed under electron microscope.Results Normal rats had clear layers of retinal structures,the inner and outer segments of rod cells were in a neat and orderly arrangement.The inner and outer nuclear layer arranged in sequence and stained evenly.Each group was observed a thinner and sparser change in the outer nuclear layer after light exposure.The outer segments of rod cells arranged disorderly,part of the membranous discs were ruptured.Swollen mitochondria of the rod cells in inner segments were also observed.Outer nuclear layer cells showed karyopycnosis and maldistribution.The changes in each dose group were improved compared with sham group.Conclusion The establishment of the retinal light injury model is successful by using the operating microscope.Each dose of Compound Light Capsules shows a protective effect on retinal light injury.