苦荞中肌醇含量的测量方法研究

Measuring Methods of Inositol Contetin Buckwheat

目的为得到一种简单、快速、准确测定苦荞中肌醇的方法.方法采用高效液相色谱-蒸发光散射检测器(HPLC-ELSD)法进行测定,色谱柱为美国Phenomenex公司提供的Luna C18柱(4.6 mm×150 mm,3μm),流动相为甲醇-水(含0.1%的冰醋酸),流速:1 mL/min.漂移管温度50℃,载气压力40 psi.结果肌醇进样量在0.0268～0.1340μg范围内(r=0.9997)线性关系良好,平均加样回收率为99.7%(相对标准偏差RSD=3.49%).结论该方法简便可靠,可用于苦荞中肌醇含量的质量控制.

Objective To explore a simple,rapid and accurate method for detecting inositol content in buckwheat.Methods HPLC-ELSD method was used to detect the content of inositol in buckwheat.A Phenomenex Luna-C18 column（4.6 mm×150 mm,3 μm）was used for separation.Methylalcohol-Water（containing 0.1% glacial acetic acid）was used as mobile phase at a flow rate of 1 mL/min.The temperature of drift tube was 50 ℃,and the gas pressure was 40 psi.Results Under the above described chromatographic conditions,the linear ranges of inositol was 0.0268～0.1340 μg（r=0.9997）,and the mean recovery rate was 99.7%（RSD=3.49%）.Conclusion The assay method is simple and accurate for the quality contro1 of inositol in buckwheat.