产ESBLs革兰阴性菌的TEM、SHV、CTX-M基因分型研究

Detection of TEM,SHV and CTX-M genotype of ESBLs-producing gram-negative bacteria

目的:检测本院临床产超广谱β-内酰胺酶(ESBLs)革兰阴性菌的TEM、SHV、CTX-M基因型特征。方法:双纸片法确定产ESBLs的临床分离菌,提取质粒DNA,PCR法扩增ESBLs的TEM、SHV、CTX-M基因片段,对临床分离的12株肺炎克雷伯菌,17株大肠埃希氏菌和16株铜绿假单胞菌,共45株革兰阴性菌进行TEM、SHV、CTX-M基因分型研究。结果:本院临床分离出的45株产ESBLs临床分离菌的质粒中,有36株扩增出TEM基因片段,33株扩增出SHV基因片段,31株扩增出CTX-M基因片段,TEM、SHV、CTX-M基因片段的检出率分别为80%、74%和68%。结论:TEM、SHV、CTX-M已成为本院临床分离产ESBLs菌的主要耐药基因型,有必要进一步加强临床耐药基因的监测,为临床用药和医院感染监控提供参考。

Objective： To detect extended-spectrum β-lactamases（ESBLs） genotyping of ESBLs-producing gram-negative bacilli.Methods： Clinical isolated ESBLs-producing strains were detected by double-disk method;TEM,SHV and CTX-M gene fragments of ESBLs were amplified by PCR.PCR was used to study SHV genotyping of 17 E.coli,12 K.pneumonia and 16 P.aeruginosa clinical isolated in Chengdou.Results： There were 36 strains TEM gene fragments,33 strains SHV gene fragments and 31 strains CTX-M gene fragments.TEM,SHV and CTX-M gene rate reached 80%,74% and 68% in clinical isolated ESBLs-producing strains.Conclusion： Genotyping of clinical isolated strains producing ESBLs,which helps to carry out clinical research and monitoring resistant genes.