摘要
蛋白质工程技术是在基因水平上通过分子设计和DNA重组技术合成自然界不存在的蛋白质的新技术。介绍定点突变、体外定向进化和tRNA介导蛋白质工程3类蛋白质工程技术,并综述其在生物药物研发中的应用。
By molecular design and DNA recombinant technique based on the gene level,protein engineering technique is to synthesize the novel protein which does not exist in nature.Three kinds of protein engineering technique,including site directed mutagenesis,direction evolution in vitro and tRNA-mediated protein engineering were introduced,and their applications in RD of biomedicines were reviewed.
出处
《药学进展》
CAS
2011年第12期529-536,共8页
Progress in Pharmaceutical Sciences
基金
甘肃省自然基金(No.1107RJZA225)
兰州理工大学科研发展基金(No.0908ZXC126)
关键词
蛋白质工程技术
生物药物
定点突变
体外定向进化
tRNA介导蛋白质工程
protein engineering technique
biomedicines
site-directed mutagenesis
direction evolution in vitro
tRNA-mediated protein engineering
