野蔷薇中齐墩果酸提取工艺的研究

Study on extraction technology of oleanolic acid in Rosa multiflora Thumb

优选野蔷薇中齐墩果酸的超声提取工艺。方法:使用超声波提取法,单因素试验,采用L(934)正交设计试验,用高效液相色谱法测定齐墩果酸的含量,确定最佳提取工艺条件。结果:最佳提取工艺条件是液料比15∶1,超声提取40min,80%vol乙醇溶液,温度50℃。结论:采用此工艺提取方法高效经济、快捷,易于推广。

To optimize the ultrasonic extraction condition for oleanolic acid from Rosa multiflora Thumb. Method： HPLC was used to analyze the content ofoleanolic acid. According to the single factor experiments, orthogonal design L9（34）, the optimization of the ultrasonic extraction technology was determined by test investigation. Results： the optimum extraction condition was： 15 fold solvent, 40min by ultrasonic extraction, 80%vol ethanol solution, temperature was 50℃. Conclusions： the optimized technology is efficient economy, quick, easy to generalize.