高抑菌活性链霉菌的原生质体制备及诱变选育

Protoplast formation of Streptomycetaceae A01 with strong antibacterial activity and mutagenesis

为了提高链霉菌A01的对金黄色葡萄球菌的抑菌活性,从酶、渗透压稳定剂、培养时间等方面研究原生质体的制备条件,并采用紫外线对制得的原生质体进行诱变。结果表明:30℃时菌丝体培养8d,以0.6mol/L甘露醇溶液配制的1%蜗牛酶+1%溶壁酶的混合酶液进行酶解,原生质体的产量最高。紫外线照射25s,抑菌圈最大,直径能达到19.1mm,抑菌活力提高了47%,且性能稳定。

In order to improve the antibacterial activity of Streptomycetaceae A01 on Staphylococcus attreus, the protoplast formation conditions, such as enzymes, osmotic stabilizer, incubation time, were studied and then the protoplast was mutated by UV. The results showed that the optimal conditions of protoplast formation were as follows： culture temperature 30℃, culture time 8d, mixed enzyme solution of 1% snailase and 1% lywallzyme with 0.6mol/L mannitol. Under these conditions, the protoplast production was the highest. By UV mutation for 25s, the diameter of inhibiting bacteria circle was up to 19.1mm, and the antibacterial activity enhanced by 47% with good stability in heredity.