基于权重配方法的降脂颗粒治疗非酒精性脂肪性肝病组分配伍优化研究

Research on optimizing components compatibility of Jiangzhi granula in NAFLD treatment

目的:依据权重配方法结合治疗非酒精性脂肪性肝病(nonalcoholic fatty liver disease,NAFLD)的药效,筛选降脂颗粒组分配比最优方案。方法:降脂颗粒中5味药的提取物,按权重配方设计表U6(35)配制成3水平5味药共6个配伍组(A、B、C、D、E、F)。雄性Wistar大鼠,随机分为空白组(K)、模型组(M)、降脂颗粒6个配伍组(A～F)和辛伐他汀组(S)。K组给予普通饲料,其余予高脂饲料。造模同时A～F和S组给予相应药物灌胃,K、M组予生理盐水,持续6周。收集各组大鼠肝组织和血清,检测血清ALT、AST、HDL、LDL和肝组织甘油三酯(TG)含量,分析肝脏组织病理学变化。结果:M组大鼠肝细胞明显脂肪变性,A～F组与S组大鼠肝组织均有不同程度改善,且A～F组优于S组。相对于K组,M组大鼠ALT、AST升高;A～F组与M组相比ALT、AST水平下调,D、E组下调趋势明显,S组无改善。M组大鼠LDL升高,C、E组LDL水平显著降低。M组大鼠肝组织TG显著升高,A～E和S组TG均显著下调,其中A、C、E组优于S组,E组作用最明显。结论:降脂颗粒各组分配伍组对大鼠NAFLD模型均有疗效,其中优选出E组疗效最显著,可作为研发治疗NAFLD疗效更优的中成药的基础。

Objective：Based on weighed modification method and therapeutic efficacy for non-alcoholic fatty liver disease（NAFLD）,this study aims to select out the optimal compatibility of Jiangzhi granula components.Methods：According to weighed modification table U6（35）,5 herbal extracts of Jiangzhi granula（gynostemma,cuspidatum,capillaris,salvia,lotus）,were combined into compatibility groups（named A,B,C,D,E,F） based on 3 does levels of 5 herbs.Male Wistar rats,randomly divided into control group（K）,model group（M）,6 Jiangzhi granula compatibility groups（A^F） and simvastatin group（S）.Rats in M and treatment group were fed with high fat diet.For A^F and S group,therapeutic interventions were administrated intragastricly in the meantime for 6 weeks.Indices of treatment efficacy including serum ALT,AST,HDL,LDL,liver TG concentration,as well as liver pathology were measured.Results：HE stain of liver tissues showed apparent hepatosteatosis in M group,which was improved more in A^F than in S group.Serum ALT,AST in M increased,while down-regulated in Jiangzhi granula combination groups,and the trend was higher in D,E group.Compared with K,serum LDL in M group was significantly higher,C,E group reduced LDL level obviously.Liver TG increased in M group,TG level all decreased in A^E and S group,and E group showed the most significant effect.Conclusion：All 6 groups of Jiangzhi granula components combination have therapeutic effect for rat NAFLD model.Especially group E has the best response,which might be the basis of developing a new Chinese medicine with better efficacy and quality guarantee.