杏花粉活力测定培养基筛选及耐贮性研究

Optimization of Culture Medium for Testing Apricot Pollen Viability and Study of the Storability of the Fruit

【目的】以固体琼脂培养基离体培养‘大黄杏’品种花粉,比较不同浓度蔗糖、硼酸对花粉离体萌发的影响。【方法】用筛选出的最适培养基测定三种贮藏温度条件下花粉活力变化。【结果】‘大黄杏’品种最适萌发培养基为:15%蔗糖+0.01%硼酸+1%琼脂;干燥密闭条件下,为期1年贮藏于三种温度条件下花粉活力大小依次均为:-18℃>4～5℃>室温,活力下降幅度由大到小则依次均为:室温>4～5℃>-18℃。【结论】花粉于-18℃贮藏后1年活力仍达30.00%,4～5℃在贮后第11个月丧失活力,而在室温下贮藏3个月花粉即失去活力。

[ Objective and Method] The pollen viability of Dahuangxing cultured in vitro on agar medium supplemented with different concentrations of sucrose and boric acid were compared, and the optimum medium was used to test the pollen vitality which was stored at three different temperatures. [ Result ] The results showed that the medium with 15% sucrose, 0.01% boric acid and 1% agar is the best for germination, and besides, stored in dry and closed condition, the pollen vigor at three diffeIent temperatures from high to low always was ： - 18 ℃, 4 - 5 ℃, room temperature, while the decrease amplitude of the pollen viability was just the opposite. [ Conclusion] The pollen viability stored at - 18℃ still could reach to 30.00% one year later, while stored at 4 - 5℃ the pollen viability would be lost after storage for eleven months, and the room temperature kept the pollen vigor only for 3 months.