斑马鱼胚胎在毒理学研究中的应用

Application of zebrafish embryo in toxicology

斑马鱼胚胎对化学品暴露高度敏感,同时具有许多其他动物模型所没有的优势,因此,在毒理学研究中得到广泛应用。除化学品浓度外,在毒性检测中还需要考虑一系列因素的影响,如胚胎膜屏障作用、胚胎暴露时间、化学品的性状及不同化学品间的相互作用。目前,胚胎毒性评价方法由表型的观察逐渐向分子水平发展,更加侧重于对化学品作用机理的研究,使斑马鱼胚胎在毒性检测中具有更高特异性和灵敏性。

Zebrafish embryo is highly sensitive to chemical exposure and has much unique superiority over other animal models, so it is widely applied in toxicology. Besides chemical concentration, many other influences, such as chorion barrier, embryo exposure time, chemical characteristics and the interaction between different chemicals, should also be considered. Assessment methods of embryo toxicity are evolving from morphologic observation to molecular research, more emphasis on chemical mechanism. This makes zebrafish embryo have higher specificity and sensitivity in the toxicity detection.