HIF-1α在膀胱移行细胞癌中的表达及临床意义

Expression and the Potential Clinical Implication of HIF - 1α in Human Bladder Transitional Cell Carcinoma

目的:检测膀胱移行细胞癌(BTCC)中HIF-1α的表达情况,初步探讨HIF-1α在BTCC中表达的临床意义。方法:应用免疫组织化学及RT-PCR技术检测BTCC和正常膀胱组织中HIF-1α蛋白和mRNA表达情况,并分析其表达与肿瘤临床分期、病理分级及复发的关系。结果:免疫组织化学染色显示,12例正常膀胱组织未发现HIF-1α阳性表达,57例BTCC中有30例HIF-1α表达阳性,阳性表达率为52.6%。其表达与肿瘤的病理分级无关,但与肿瘤的临床分期及复发相关。RT-PCR检测显示,BTCC中HIF-1αmRNA的阳性表达率高于正常膀胱组织,差异具有统计学意义,但BTCC中HIF-1αmRNA的表达与其蛋白水平表达无明显相关性。结论:在BTCC中,HIF-1α的表达明显上调,可能在BTCC的侵袭等生物学过程中具有重要的作用。

Objective： To investigate the clinicopathological significance of HIF-1α expression in bladder transitional cell carcinoma（BTCC）.Methods： HIF-1α protein and mRNA expressions were examined using immunohistochemistry and reverse-transcriptase polymerase chain reaction（RT-PCR）,respectively.The relationship between HIF-1α expression and various clinicopathological parameters including pathological grade and clinical stage was analyzed.Results： Immunohistochemically,over-expression of HIF-1α was frequently（30 of 57 cases,52.63 %） detected in BTCC tissues,whereas no HIF-1α expression was found in 12 normal bladder tissues.HIF-1α expression was significantly associated with invasive bladder cancer（Tis-T1 versus T2-T4,P =0.026）,but not with increased pathological grade.With RT-PCR technology,the positive expression rate of HIF-1α and mRNA in BTCC group was higher than that in the normal bladder tissues group（P = 0.033）.There was no correlation between the expression of HIF-1α protein and the HIF-1α mRNA expression.Conclusion： HIF-1α protein is frequently expressed in BTCC,HIF-1α may play a critical role in the invasion of BTCC and can be used to estimate its progression.