摘要
根据GenBank公布的香菇杆形病毒部分基因组序列(登录号:GQ372842)设计引物,建立了香菇菌丝体病毒的反转录-聚合酶链式反应(reverse transcription polymerase chain reaction,RT-PCR)检测方法,浙江省的36个香菇栽培菌株的菌丝体经鉴定,菌株988、939、937、9319、868、66和WL-3的菌丝体不含杆形病毒;菌株灵的菌丝体可能含有杆形病毒;另外28个香菇菌株的菌丝体可能感染杆形病毒。
Primers designed according to the partial genome sequence （GenBank Accession No.- GQ372842） of a rod-shaped virus present in Lentinula edodes mycelium were used to develop a RT-PCR-based viral detection method. Tests conducted on the mycelium of 36 L. edodes cultivars from Zhejiang Province revealed that strains 988, 939, 937, 9319, 868, 66 and WL-3 were virus-free. Mycelium of 28 other test strains contained the rod-shaped virus but it was unclear if the virus was present in mycelium of L. edodes Ling strain.
出处
《食用菌学报》
北大核心
2011年第3期79-82,共4页
Acta Edulis Fungi
基金
中国科学院知识创新工程重要方向项目(编号:KSCX2-YW-Z-0938)
浙江丽水市科技合作项目(编号:20080409)的部分研究内容
关键词
香菇
菌丝体
杆形病毒
反转录-聚合酶链式反应
Lentinula edodes
fungal mycelium
rod-shaped virus
reverse transcription polymerase chain reaction
