银杏叶提取物预防应激性胃溃疡作用机制分析

The effect and mechanism of ginkgo biloba extract on stressinduced gastric ulceration in rats

目的：探讨银杏叶提取物（ＥＧｂ）对大鼠应激性胃溃疡发生的保护作用及其机制。方法：雄性Ｗｉｓｔａｒ大鼠，随机分成对照组和ＥＧｂ 处理组；以冷拘束法建立应激性溃疡模型，通过胃窦部埋置电极记录应激状态胃平滑肌自发电活动的变化，并测定血浆和胃粘膜组织丙二醛（ＭＤＡ）含量和胃粘膜的病理改变。结果：ＥＧｂ１０～４０ ｍ ｇ／ｋｇ预处理可显著压抑应激所致的胃肌电活动紊乱及血浆和胃粘膜组织ＭＤＡ 水平的异常升高（Ｐ＜ ０．０５ 或Ｐ＜ ０．０１），使胃粘膜溃疡指数明显降低（Ｐ均＜ ０．０５），并具有剂量依从性。结论：ＥＧｂ 对冷束缚应激引起的大鼠胃粘膜损伤有明显的保护作用，其机制可能是通过改善胃平滑肌动力障碍和清除自由基来实现的。

Objective:To investigate the protective effects and mechanism of ginkgo biloba extract (EGb) on stressinduced gastric dysfunction and mucosal lesions in rats.Methods:Male Wistar rats were randomly assigned to control group and EGb treated group.The stress ulceration was produced by cold restraint,and gastric myoelectric activity was recorded during stress by implanting electrodes in the stomach antrum smooth muscle.The malondialdehyde(MDA) concentrations in plasma and gastric mucosa tissue were measured,and gastric mucosal lesions were examined in rats.Results:Pretreatment with EGb (1040 mg/kg) not only significantly inhibited the myoelectric activity response,but significantly reduced MDA levels and gastric mucosal ulceration index induced by stress in a dosedependent manner ( P <0 05 and P <0 01 vs.control group).Conclusions:EGb has protective effect on gastric movement dysfunction and mucosal lesions induced by cold restraint stress.The potential mechanism may be associated with modulation of gastric myoelectric activity and reduction of free radicals by treatment with EGb.