肺孢子菌感染大鼠血清同型半胱氨酸变化分析

Analysis on HCY Changes in Serum of Rat Infected with Pneumocystis Carinii

目的探讨肺孢子菌肺炎(PCP)的发病机制.方法纯系Wistar雌性大鼠36只随机分为PCP模型组(P组12只)、TMP-SMZ治疗组(S组14只)及正常对照组(N组10只).P组及S组于大鼠后腿肌注地塞米松(每次1mg/只,每周2次、间隔3d),诱导建立PCP动物模型.N组同法肌注生理盐水.镜检确认PCP诱导成功后,S组从第3周起每天灌胃TMP-SMZ 1次[250mg/(kg.d),5d为1疗程,以后每隔2周,1个疗程],共3个疗程.观察肺泡内肺孢子菌(PC)情况,检测血清中同型半胱氨酸(Hcy)水平,并与对照组(N组)比较.结果造模成功后Hcy测定结果逐渐增高,P组在第12周的(11.28±1.54)g/L显著高于N组的(9.46±1.34)g/L(P<0.01),S组虽也有增高趋势,但第12周(10.58±1.97)g/L与N组(9.46±1.34)g/L比较差异无显著性(P>0.05).P组第9周(10.85±1.33)g/L、12周(11.28±1.54)g/L显著高于0周(9.38±1.66)g/L,而S组间差异无显著性(P>0.05).P组与S组间,Hcy差异无显著性(P>0.05).结论 PCP造模成功后,血清中Hcy显著增高,提示血清中Hcy浓度可作为PCP感染的微血管病变的辅助诊断指标.

Objective To investigate the pathogenesis on Pneumocystis carinii pneumonia （PCP）. Methods 36 female Wister rats were randomly divided into normal group （N group）, PCP model group （P group） and TP-SMZ treatment group （S group）. For rats of P and S groups, 25 mg/Kg dexamethasone was injected in their leg muscle twice a week. H20 was injected to rats of N group in the same way to make PCP animal model. From the third week, S group rats, were infused with 250 mg/kg d for 5 days as a treatment course. In the experiment, infusing treatment course was done once for two weeks and three courses altogether, sICAM-1 in serum, pathological changes in the lung and liver and Pc in alveoli of lung were observed. And also pneumocystis carinii （P. C） in alveolar was observed, serum levels of homocysteine （Hcy） were tested and the results wered compared with that of control group （N group）. Results Hcy levels （11.28 ±1.54） g/L gradually increased after the animal model was constructed, which were higher notably than that in group N （9.46± 1.34） g/L （P〈0.01） in 12th week. Though the level （10.58±1.97） g/L tended to increase in S group, there was no significant difference compared with that of Ngroup （9.46±1.34） g/L in 12thweek. The results of Pgroupatgth （10.85±1.33） g/L and 12th week （11.28±1.54） g/Lwere higher notably than that at 0 week （9.38±1.66）g/L（P〈0.05, P〈0.01）. There was no significant difference in S group （P〉0.05）. There was no significant difference （P〉0.05） between P group and S group in Hcy levels. Conclusions After construction of PCP, the serum Hcy levels were significantly increased and it suggested that serum Hcy concentrations were diagnostic marker of microvascular disease for PCP infection.