摘要
采用原生质体融合-紫外诱变技术分离得到高效絮凝剂产生菌Enterobacter sp. W16-c,并对其产絮特性和影响因素进行分析。结果表明:Enterobacter sp. W16-c的絮凝能力比出发菌提高13.91%;突变菌株发酵产絮最适碳源为蔗糖,最适氮源为酵母膏,最佳初始发酵pH值为5～9;金属离子Fe2+、Na+对Enterobacter sp. W16-c的产絮效果有促进作用;在最佳发酵条件下所产絮凝剂的絮凝率可达96.2%;Enterobacter sp. W16-c的主要成分为多糖;Enterobacter sp. W16-c是一株产絮性能稳定的高效产絮突变菌株。
A bioflocculant production mutant Enterobacter sp. W16-c was screened by protoplast fusion-UV mutation, and its flocculation characteristics and influence factors were analyzed. The results show that the flocculation rate of mutant W16-c is increased by 13.91% compared with the orlginal strain. The optimal substrates for mutant Enterobacter sp. W16-c are su- crose as carbon source, yeast extract as nitrogen source when pH = 5-9. Metal irons Fe2＋ and Na~ could promote the biofloc- culant production by mutant Enterobacter sp. W16-c. At optimal fermentation condition, the floeculation rate of mutant W16- c could reach as high as 96.2%. The main components of the mutant W16-c are polysaccharides. The mutant W16-c is a stable and efficient biofloceulant production bacterium.
出处
《中国石油大学学报（自然科学版）》
EI
CAS
CSCD
北大核心
2011年第6期164-168,共5页
Journal of China University of Petroleum(Edition of Natural Science)
基金
中石油科学研究与技术开发项目((06A0302)
关键词
生物降解
突变
微生物絮凝剂
絮凝率
原生质体融合
biodegradation
mutagenesis
bioflocculant
flocculation rate
protoplast fusion
