外源性磷酸肌酸钠对肢体缺血再灌注的保护作用

Protective Effects of Creatine Phosphate on Ischemia and Reperfusion Injury of Limbs

目的探讨外源性磷酸肌酸钠(CP)对缺血再灌注肢体的保护作用。方法健康成年家兔20只,随机分为对照组、缺血再灌注组(IR)、低浓度磷酸肌酸钠组(LCP)和高浓度磷酸肌酸钠组(HCP),每组5只。制备兔左后肢缺血再灌注模型。静脉注射生理盐水和不同浓度的CP,再灌注前、后不同时间取血测定血清乳酸脱氢酶(LDH)、天冬氨酸氨基转移酶(AST)、丙二醛(MDA)的含量及超氧化物歧化酶(SOD)活力。电镜观察肌细胞超微结构变化。结果缺血再灌注后LCP和HCP组LDH、AST、MDA含量低于IR(P<0.05),SOD活力显著高于IR(P<0.01);电镜下LCP和HCP组肌损伤轻于IR组。结论外源性磷酸肌酸钠能够保护缺血再灌注骨骼肌。

Objective To study the protective effect of creatine phosphate on ischemia reperfusion of skeletal muscle. Methods The experimental models of ischemia referfusion injury in extremities were produced in 20 healthy adult rabbits,which were randomly divided into control,reperfusion,low concentration and high concentration with 5 rabbits in each .Isolated left hindlimb of rabbits were used as ischemia-reperfusion models.Normal saline injection, Creatine phosphate 0.Sg and lg were infused intravenously into the three groups just before blood reperfusion. At the points of pre-ischemia,1 and 4 hours after reperfusion,the blood samples were collected re- spectively for measurements of LDH,AST, MDA and SOD. Meanwhile,the tibialis anterior muscles were harvested for examination of the micro-structure and the uhrastructure by using electron microscope. Results After repeffusion,the contents of AST,LDH,MDA signifi- cantly decreased（P〈0.05）and the activity of SOD obviously increased in the HCP and LCP groups compared with reperfusion group（P〈 0.01 ）. The electron microscope examinations showed severe tissue injury in the reperfusion group but mild in HCP and LCP groups. Conclusion Creatine phosphate has a significant protective effect on ischemia reperfusion injury in extremities.