延藿骨宝与己烯雌酚对去卵巢大鼠骨代谢影响的定量研究

Quantitative Analysis Study of the Effects of Chinese Herbal Medicine Yanhuogubao and Diethylstilbestrol on Bony Metabolism of Ovariectomized Rats

目的 了解中药复方制剂延藿骨宝与己烯雌酚(DES)对去卵巢大鼠骨代谢的影响,并对两者疗效进行比较。方法 用3月龄雌性SD大鼠31只,随机分为基础对照组、年龄对照组、去卵巢DES治疗组〔灌胃45mg/L的己烯雌酚5ml/(kg·d)〕和去卵巢延藿骨宝治疗组〔灌胃含生药浓度为330g/L的延藿骨宝5ml/(kg·d)〕,每周6次,12周后处死,胫骨上段行不脱钙骨制片骨形态计量学分析。结果 己烯雌酚与延藿骨宝都有预防去卵巢大鼠骨质疏松的作用(骨小梁面积百分率分别增加了122%和83%),但两者作用机理不同,己烯雌酚主要是明显抑制去卵巢引起的骨高转换,而延藿骨宝则主要是抑制去卵巢大鼠的骨吸收,对骨形成无明显抑制作用。结论 延藿骨宝与己烯雌酚都有预防去卵巢大鼠骨丢失的作用,两者效应基本一致。

Objective To compare the effects of Chinese Herbal Medicine Yanhuogubao and diethylstilbestrol(DES) on bony metabolism of ovariectomized (OVX) rats.Methods 31 3 month old SD female rats were randomly divided into five groups:the basal control,the age control,the OVX,OVX with DES(5 ml/ (kg·d), DES:4.5mg/L),and OVX with Chinese Herbal Medicine Yanhuogubao (5ml/(kg·d),crude drugs:330g/L).All rats were killed at the 12th week.The proximal tibia of rats were processed its undecalcified section of 20μm thickness for bone histomorphometric analysis.Results DES inhibitied the high bone turnover of OVX rats,but Chinese Herbal Medicine Yanhuogubao mainly inhibitied bone resorption and had no obvious inhibition to bone formation.Conclusion Both DES and Yanhuogubao have effects on preventing bone loss in OVX rats.