RP-HPLC法同时测定叶下珠中没食子酸、柯里拉京和短叶苏木酚的含量

Simultaneous determination of gallic acid,corilagin and brevifolin in Phyllanthus urinaria by RP-HPLC

目的建立反相高效液相色谱法同时测定叶下珠中没食子酸、柯里拉京和短叶苏木酚的含量。方法采用Kromasil C18色谱柱(250 mm×4.6 mm,5μm),流动相为乙腈-体积分数0.1%磷酸水溶液梯度洗脱,流速1.0 mL.min-1,检测波长为280 nm,柱温30℃。结果没食子酸、柯里拉京和短叶苏木酚的质量浓度分别在5.300～106.7 mg.L-1(r=0.999 8)、2.00～40.6 mg.L-1(r=0.999 7)、0.270～5.42 mg.L-1(r=0.999 7)内与峰面积呈良好的线性关系。没食子酸、柯里拉京和短叶苏木酚的平均加样回收率(n=9)分别为97.9%、101.7%、100.2%,RSD分别为1.8%、1.8%、2.7%。结论该分析方法准确可靠,重复性好,为更好地控制叶下珠药材的质量提供方法。

Objective To establish an HPLC method for simultaneous determination of gallic acid, corilagin and brevifolin in Phyllanthus urinaria. Methods The chromatographic separation was achieved on a Kromasil C18（250 mm ×4. 6 mm,5 μm）column with acetonitrile-0. 1% phosphoric acid solution as mobile phase （gradient elution）. The flow rate was 1.0 mL·min^-1 ,the column temperature was maintained at 30 ℃ ,and the detective wavelength was set at＇ 280 nm. Results The calibration curves of gallic acid, corilagin and brevifolin were linear in the range of 5. 300- 106.7 mg·L^-1 （ r =0. 999 8） ,2. 00 -40. 6 mg·L^-1 （ r =0. 999 7）, 0. 270-5.42 mg·L^-1 （ r = 0. 999 7 ）, respectively. The average recoveries （ n = 9 ） were 97.9%, 101.7%, 100. 2% ;and the RSDs were 1. 8%, 1.8% ,2. 7%, respectively. Conclusions The method is reliable, accu- rate and reproducible for quality control of P. urinaria.