人肺表面活性物质结合蛋白A基因组克隆及亚克隆

Cloning and Subcloning of Chinese Pulmonary Surfant Protein A.

【目的】 获得ＳＰＡ Ⅰ基因克隆及亚克隆，并进行序列分析，为研究ＳＰＡ 基因结构、表达调控、基因工程生产奠定基础。【方法】 以正常人血白细胞染色体ＤＮＡ 为模板，采用ＰＣＲ 及基因克隆技术，获得ｐＢＳ／ＳＰＡ Ⅰ克隆和ｐＵＣ１９／６３０ｂｐ 亚克隆，测序证实之。【结果】 测序结果显示，与已发表的ＳＰＡ Ⅰ序列相比，未发现基因突变。【结论】 证实获得的基因为ＳＰＡ Ⅰ。

Objective To get clone and subclone of Chinese pulmonary surfactent protein A and sequence it,which will be the base of the further study in SP A gene structure,expression control and genetic engineering. Methods pBS/SP A Ⅰ and pUC19/630bp clone was got from the normol Chinese chromosome DNA in WBC by PCR and gene cloning,and proven with measuring the sequence. Results Compared with SP A Ⅰ sequence that was published,there is no mutation. Conclusion :SP A clone was got.