应用巢式RT-PCR检测原发性肝细胞癌患者外周血中的微转移

Detection of peripheral blood micrometastasis in patients with hepatocellular carcinoma by nested RT-PCR

目的 以AFPmRNA为标志基因 ,检测原发性肝细胞癌患者外周血中微转移情况。方法 应用巢式RT PCR方法 ,检测 71例肝细胞癌、2 1例肝硬化患者、2 8例健康对照、4例转移性肝癌、4例肝脓肿、4例肝血管瘤及 5例其它恶性肿瘤患者外周血中AFPmRNA的表达。结果 71例肝癌患者中 ,AFPmRNA阳性 3 9例 ,阳性率为 5 4.9% ,其中 8例有远处转移的全部阳性 ( 10 0 % ) ,未发现远处转移者阳性 3 1例 ,阳性率为 49.2 %。 2 1例肝硬化患者中仅 2例阳性 ,而转移性肝癌、肝血管瘤、肝脓肿、其它恶性肿瘤、健康对照均为阴性。结论 检测肝细胞癌患者外周血中的AFPmRNA ,是一种早期发现肝细胞癌血道播散的灵敏方法。

? Objective To detect hepatocyte associated gene AFPmRNA to demonstrate micrometastasis in peripheral blood.Methods AFPmRNA in peripheral blood was detected in 71 case with PHC,21 cases with cirrhosis,28 normal healthy controls,4 cases with metastatic liver cancer,4 cases with liver hemangioma,4 cases with liver abscess and 5 cases with other maligant diseases by means of nested reverse transcriptase polymerase chain reaction.Results Human AFPmRNA was detected in 39 out of 71 cases of PHC(54.9%),in which 8 patients with distal metastasis were all positive and in 31 without distal metastasis the positive rate was 49.2% (P<0.01).AFPmRNA was also positive in 2 cases of cirrhosis (2/21,9.5%),but negative in normal healthy control,metastatic liver cancer,liver hemangioma,liver abscess and other maligant disease.Conclusions Nested reverse transcriptase polymerase chain reaction is a very sensitive method for early detecting micrometastasis in patients with PHC,and suitable for extensive clinical application. 〔