原发性非小细胞肺癌P16基因的缺失及其临床意义

Deletion of P16 gene in non-small cell lung cancer

目的 探讨P16基因缺失与非小细胞肺癌病理类型、临床分期的关系。方法 采用聚合酶链反应 (PCR)技术 ,检测 3 1例非小细胞肺癌及相应匹配癌旁正常组织中P16基因外显子 2的缺失情况。结果 3 1例非小细胞肺癌组织中 ,7例P16基因纯合性缺失 ,缺失频率为 2 2 .6 %。其中鳞癌 6例 ,腺癌 1例。 7例缺失均为Ⅲ、Ⅳ期临床病例。结论 P16基因缺失可能在非小细胞肺癌的发生、发展中起作用 ,并与病理类型、临床分期有关。

? Objective To investigate the possible relationship between deletion of P16 gene and cellular type、clinical stages of non small cell lung cancer.Methods A total of 31 non small cell lung cancer tissue specimens and matched normal lung tissue was examined for deletion of P16 gene by polymerase chain reaction analysis.Results The homozygous deletion of the P16 gene in seven samples were confired by PCR with a 22.6% rate of P16 gene deletion,including 6 squamous carcinormas、1 adenocarcinomas.The seven patients with homozygous deletions were in stage Ⅲ or Ⅳ.Conclusions These data suggest that P16 gene alterations may play a role in the progression of human non small cell lung cancer and have relationship with cellular type and clinic stages. 〔