应用流式细胞技术检测COPD患者外周血中Th17细胞的变化及临床意义

目的研究慢性阻塞性肺疾病(COPD)患者外周血中Th17细胞的变化及临床意义。方法选择COPD住院患者30例作为观察组,同时设置健康对照组30例,利用流式细胞技术分别检测对照组及观察组患者急性期、缓解期外周血中Th17细胞数量的动态变化。结果 COPD患者外周血中Th17细胞数量增高,与疾病活动程度呈正相关;急性期高于缓解期高于正常对照。结论外周血中Th17细胞在COPD中有数量的增多和动态变化,为该疾病的临床诊断、治疗提供理论和实验依据。

Objective To study the concentration variation and clinical significance of Th17 in peripheral blood of COPD patients. Methods 30 hospitalized patients with COPD were selected as a study group, and while setting the healthy control group of 30 patients, the Thl 7 cells number in peripheral blood and IL-17 level dynamic changes of the control group and observation group patients with acute, remission were detected by flow cytometer method. Results The 17 cells in peripheral blood of patients with COPD the number of secretion increased with disease activity was positively correlated; acute phase was higher than remission phase, and higher than normal controls. Conclusion Th17 cells in peripheral blood of COPD patients has increased the number and dynamic changes, can provide a theoretical and experimental basis for the clinical diagnosis and treatment of COPD.