HBV携带产妇血清及乳汁中HBV-DNA含量检测及其临床意义

Detection of HBV-DNA content in serum and breast milk of parturient women carrying HBV and its clinical significance

目的:探讨HBV携带产妇的血清及乳汁中HBV-DNA含量,以期指导母乳喂养。方法:选取HBV携带产妇100例,采用荧光定量PCR技术检测其血清、乳汁中HBV-DNA含量。结果:HBsAg、HBeAg双阳性组和HBsAg单阳性组中血清HBV-DNA含量、阳性率均高于乳汁,差异有统计学意义(P<0.05),且乳汁HBV-DNA阳性率随着母血清HBV-DNA含量的增加而增加;100例产妇中乳汁HBV-DNA检测阴性及血清病毒含量<104copy/ml的母乳喂养儿未见婴儿发生乙肝病毒感染。结论:HBV携带产妇乳汁具有一定的传染性,但乳汁的传染性低于血液,血清HBV-DNA阳性产妇在哺乳时应进行HBV-DNA检测,以保证婴儿的安全哺乳环境。

Objective： To explore the contents of HBV - DNA in serum and breast milk of parturient women carrying HBV, in order to direct breast feeding. Methods： 100 parturient women carrying HBV were selected, fluorescence quantitative PCR technique was used to detect the contents of HBV - DNA in serum and breast milk. Results： In positive HBsAg ＋ positive HBeAg group and positive HBsAg group, the contents and positive rates of HBV - DNA in serum were significantly higher than those in breast milk （P 〈 0. 05） . The positive rate of HBV- DNA in breast milk increased with the increase of HBV- DNA content in maternal serum; 100 infants who were born by the parturient women with negative HBV - DNA in breast milk and serum viral content 〈 104 copy/ml were not found with HBV infection. Conclusion： The breast milk of parturient women carrying HBV have infectivity, but the infectivity of breast milk is lower than that of serum, the parturient women with positive HBV - DNA in serum should receive HBV - DNA detection when they suckle their infants to ensure a safe lactation environment of infants.