库存全血凝血因子Ⅷ改变的研究

Study of the fuctor Ⅷ change in resevered blood

的：探讨凝血因子Ⅷ活性（ＦⅧ：Ｃ）在库血存放过程中的改变，以保证高质量采血和输血。方法：采用一期法以进口标化ＦⅧ作为参照，对存放不同时间的库血进行ＦⅧ：Ｃ测定。结果：ＦⅧ：Ｃ于４℃存放２４ ｈ，ＦⅧ：Ｃ改变不明显，至３６ ｈ，ＦⅧ：Ｃ迅速下降至原水平的４０％ ～６０％ 。而在原水平的３０％ ～４０％ 持续较长时间。结论：从止血与凝血方面而言，输入最新鲜的血液最为理想，其次为存放＜ ２４ ｈ 的全血。因子Ⅷ活性改变的观察，为制备成分输血制剂的最佳时机提供了依据。

Objective:Exploring the FⅧ activity changes during blood storage to keep high quality blood collection and transfusion.Method:FⅧ:C in stored blood was detected at different time by one stage method with import standlied FⅧ as control.Result:No obvious change was found in 4℃ stored blood for 24 h. Up to 36h,FⅧ:C decreased dramatically to 40%～60% of primary level,than,susteined at 30%～40% of primary level for a long time.Conclusion:Fresh blood (stored in 24h) is reasonable for fractioned blood tranfusion. Observation of FⅧ activity may provide the prove for choice of reasonable stored blood for fractioned blood transfusion.