Fas抗原与可溶性Fas在急性淋巴细胞白血病中的表达

Study of Fas antigen and soluble Fas expressing in acute lymphocytic leukemia

目的：研究Ｆａｓ和可溶性Ｆａｓ 在急性淋巴细胞白血病（ＡＬＬ）中的表达，探讨Ｆａｓ在白血病细胞凋亡障碍中的作用。方法：分别用免疫组化ＳＡＢＣ法和双抗夹心ＥＬＩＳＡ 法，检测３６ 例ＡＬＬ患者和１０ 例正常对照组骨髓细胞Ｆａｓ 抗原（ｍ Ｆａｓ）表达以及血浆可溶性Ｆａｓ（ｓＦａｓ）的水平。用半定量逆转录－聚合酶链式反应（ＲＴ－ＰＣＲ）检测１２ 例ＡＬＬ患者和７ 例正常对照骨髓细胞Ｆａｓｍ ＲＮＡ 的表达。结果：３６ 例ＡＬＬ初诊组患者Ｆａｓ 抗原的表达较正常对照组低〔（６．２３±６．５８）％ｖｓ （２８．６３±５．０２）％ ， Ｐ ＜ ０．００１〕，血浆中ｓＦａｓ 水平也较正常对照组低〔（１１．８ １７６±４．７ ６２０）ｎｇ／ｍ ｌｖｓ （２７．２ ８７４±９．２ ７７８） ｎｇ／ｍ ｌ， Ｐ＜ ０．００１〕。ＡＬＬ组未见Ｆａｓ ｍ ＲＮＡ经转移剪接后缺乏跨膜区的１ １０４ ｂｐ（对应于ｓＦａｓ）条带，而正常对照组可见；ＡＬＬ组Ｆａｓ ｍ ＲＮＡ的表达较正常对照组也显著降低（０．３ ４０７ ｖｓ ０．８ ３０３， Ｐ＜ ０．００１）。结论：Ｆａｓ 可能参与了白血病细胞的凋亡障碍，ＡＬＬ白血病细胞可能不是通过提高血浆ｓＦａｓ 水平来逃避凋亡。白血病细?

Objective:To study the expression of Fas and soluble Fas in acute lymphocytic leukemia (ALL) in order to elucidate its role in the failure of apoptosis in leukemias.Method:Strept avidin biotin complex method (SABC) and ELISA were respectly used to detect the expression of Fas antigen (mFas) in bone marrow cells and that of soluble Fas (sFas) in plasma in 36 ALL patients and 10 normal volunteers.Semi quantitative reverse transcriptase polymerase chain reaction (RT PCR) was used to detect the expres sion of Fas mRNA in 12 ALL patients and 7 normal volunteers.Result:The level of mFas in 36 newly diagnosed patients was significantly lower than that in the normal group 〔( 6.23 ± 6.58 )% vs ( 28.63 ± 5.02 )%,P< 0.001 )〕.The level of sFas in the plasma of ALL patients was also lower than that in the normal group ( 11.8 176 ± 4.7 620 ng/ml vs 27.2 874 ± 9.2 778 ng/ml,P< 0.001 ).The 1 104bp Fas △ TM cDNA band was not seen in ALL patients,but it was seen in the normal group.The expression of Fas mRNA in ALL patients was also lower than that in the normal group ( 0.3 407 vs 0.8 303 ,P< 0.001 ).Conclusion:The down regulation of Fas expression was related to the failure of apoptosis in leukemia.Leukemic cells were not certain to evade apoptosis by enhancing the level of sFas in plasma to block the Fas Fas ligand system.Fas in leukemic cells was regulated in the level of gene.