四氯化碳诱导鼠肝组织血管病变的动态观察

Dynamic observation on vascular diseases of liver tissues of rats induced by CCI_4

目的研究CCl_4诱导鼠肝内血管病变的动态变化及其在肝纤维化(LF)及肝硬变(LC)病变中的意义。方法 SD(?)6月龄鼠50只,随机分成2组,40只用于造模,10只为对照。实验鼠以剂量3mL/kg,皮下多点注射,500mL/LCCl_4(用液状石蜡配制),2次/wk,对照组不处理。分别于实验2,5,8,12wk解剖造模鼠10只,作冰冻及石蜡切片观察。对照鼠方法同上。结果实验2wk鼠体质量略下降而肝质量增加,5wk始肝质量下降,至8wk～12wk最明显(P<0.01),且肝表面伴结节形成,组织学证实已发生LC。在LC的发展中肝内血管病变起引爆和桥梁作用,通过血管炎症-血管阻塞-血管增生和改建-血管纤维化而最终形成纤维带,致肝组织血液循环障碍引发肝实质变性坏死。结论肝内血管病变是导致LC及肝细胞病变的关键因素。

AIM To study the dynamic changes of intrahepatic vascular diseases on rats induced by CCl4 and their significance in liver fibrosis (LF) and cirrhosis (LC). METHODS Fifty male 6 months old SD rats were ramdomly divided into 2 groups: 40 were used to make model (experimental group) and 10 served as controls. The experimental rats were injected with 3 mL kg of 500mL/L CCl4 (prepared with liquid paraffin) on several spots, twice a week and the control group was not treated with CCI4. Ten rats of experimental rats were studied separately on week 2, 5, 8 and 12 after experiment and frost and paraffin slices were made for observation. The control group was treated with the same way. RESULTS The quality of the bodies of experimental rats decreased slightly and that of livers increased at week 2, which began to decrease at week 5, and most significantly until week 8-12 (P<0.01). Nodules were formed on the surface of livers, and LC occurred which was conformed histologically. The intrahepatic vascular diseases played igniting and bridging roles in the development of LC. Through vascular inflammation-vascular obstruction-vascular proliferation and rebuilding-vascular fibrosis, fibrous zone was finally formed, causing disturbance of blood circulation of liver tissues, and leading to liver parenchyma denaturalization and necrosis. CONCLUSION Intrahepatic vascular diseases are the key factors which result in LC and diseases of hepatocytes.