实验性肝细胞癌铁铜含量与MR成象的关系

Relationship between metal iron, copper content and signal intensity and MR imaging of hepatocellular carcinoma in rats

目的初步阐明在MRI常规SE序列,铁、铜元素对肝细胞癌TIWI信号的影响。方法 20g/L DEN灌喂Wistar大鼠(n=80),诱癌成功后行常规SE序列T1WI,T2WI扫描,对照组(n=20)不灌喂DEN。扫描后处死动物取肝组织与肝癌组织作HE、普鲁士蓝染色,并测定组织内水、铁、铜含量。结果肝癌与癌周组织含水量无显著差异,肝癌内铁含量低于癌周肝组织,肝癌内的铜含量高于癌周肝组织,在呈低信号的肝癌组织内含铁血黄素明显高于其他信号强度类型,在呈高信号的肝癌组织内明显高于其他信号强度类型的肝癌组织。结论水的含量不是影响肝癌MR表现的主要因素,肝癌内含铁血黄素在SE T1WI呈低信号起作用,肝癌内铜的蓄积对SE T1WI呈高信号起作用。

AIM To investigate the relationship between the metal content of hepatocellular carcinoma (HCC) and the signal pattern on magnetic resonance (MR) images. METHODS Wistar rats (n = 80) were examined on SE T1WI and T2WI in rats with experimental hepatocellular carcinoma induced by 0.2% diethynitrosamine. After MR examination, rats were killed and the quantities of water, iron and copper in the tissues of HCCs and surrounding hepatic parenchyma were determined. RESULTS There was no significant difference of water content between HCCs and hepatic parenchyma, the average iron content of HCCs was lower than surrounding parenchyma but the incidence of positive peris stains in HCCs with low-intensity pattern was higher than other patterns. The incidence of copper accumulation was statistically higher in HCCs with high-intensity pattern than those with other patterns.CONCLUSION The water content is not the primary factor for HCCs in MR appearance; hemossiderin prolongs the T1 value of HCCs on SE T1WI; and copper accumulation might be a cause of high-intensity pattern on SE Tl Wl images of HCCs.