赤潮藻电致化学发光分子探针检测系统的构建

Construction of Electrochemiluminescence System for Harmful Algae Detection

为建立一种赤潮藻电致化学发光分子探针检测新技术(electrochemiluminescence-molecular probe,ECL-MP),本课题组根据电致化学发光(electrochemiluminescence,ECL)检测微量物质的原理及相关文献,自行构建了一台ECL检测装置,并对其工作性能及最适工作条件进行反复测试.结果表明,该装置能够正常工作,在电压1.0 V,电流1.0 mA,TPrA浓度1.5 mol.L-1及磷酸盐缓冲液pH 7.4的条件下对Ru(bpy)3Cl2.6H2O达到最优检测,检测限为10-11mol.L-1,线性分析范围10-9～10-5mol.L-1,可检测的物质的量范围0.4 pmol～4 nmol,跨越5个数量级.该装置灵敏度高,工作稳定,为建立赤潮藻ECL-MP技术打下了坚实的基础.

To develop a new technique, Electrochemiluminescence-Molecular Probe （ECL-MP）, for harmful algae detection, a highly sensitive electrochemiluminescence （ECL） detection system was set up based on the principle of ECL and related literature. The optimization tests were carried out, including the impact of voltage and current, the concentration of tripropylamine （TPrA） and the pH of phosphate buffer （PBS）. The determination limit of Ru（bpy）3Cl2·6H2O was 10-11 mol·L-1 and the detection range was from 10-9-10-5 mol·L-1as well as the detection amount of substance was in the range from 0.4 pmol-4 nmol in the optimal reaction conditions with voltage of 1.0 V, current of 1.0 mA, TPrA concentration of 1.5 mol·L-1 and pH of 7.4. It was proved by facts that this ECL analyzer was stable and highly sensitive, which was helpful in establishment of ECL-MP.