邻二氮菲分光光度法测定盐酸氯丙嗪

Spectrophotometric determination of chlorpromazine hydrochloride by 1,10-phenanthroline

在HAc-NaAc缓冲介质中,盐酸氯丙嗪(CPZ)能定量使Fe(Ⅲ)还原为Fe(Ⅱ),还原生成的Fe(Ⅱ)与邻二氮菲反应生成稳定的红色络合物,并且在一定范围内,CPZ的浓度和生成的红色络合物的吸光度呈良好的线性关系。据此,提出邻二氮菲-Fe(Ⅲ)体系测定盐酸氯丙嗪的新方法。在优化的实验条件下,盐酸氯丙嗪的质量浓度在0.040～15.00 mg/L范围内与吸光度呈现良好的线性关系,线性相关系数R=0.9995,摩尔吸光系数ε=2.8×104 L.mol-1.cm-1,检出限为0.020 mg/L。11次重复测定的相对标准偏差小于2.9%。该方法用于药物中盐酸氯丙嗪的测定,结果满意。

A new spectrophotometric method for the determination of chlorpromazine hydrochloride in pharmaceuticals was proposed.The developed method was based on the redox reaction of chlorpromazine hydrochloride and Fe（Ⅲ） in the HAc-NaAc buffer solutions.The reaction product Fe（Ⅱ） can form a red colored product with 1,10-phenanthroline.Under the optimum conditions,the absorbance was proportional to the concentration of chlorpromazine hydrochloride in the range of 0.040～15.00 mg/L and the detection limit was 0.02 mg/L.The molar absorptivity was 2.8×104 L·mol-1·cm-1.The relative standard deviation of 11 replicate measurements was less than 2.9%.The proposed method has been applied to the determination of chlorpromazine hydrochloride in pharmaceuticals.