摘要
采用水蒸汽蒸馏法提取浙贝母精油,GC/MS分析法结合保留指数分析法鉴定精油化学成分,琼脂扩散法检测精油的抑菌活性。首次鉴定出浙贝母精油的15种化学成分,占精油总离子流色谱峰面积的97.48%,主要成分为正十六酸(53.46%),(E,E)-9,12-十八烷二烯酸甲酯(26.96%)和油酸(9.34%)。浙贝母精油对金黄色葡萄球菌和大肠杆菌均无抑制效果,对白色念珠菌有中等抑制作用。
The essential oil from dried bulbs of Fritillaria thunbergii Miq. obtained by hydrodistil lation is analyzed by GC-MS together with Kovats retention index. The results show that major constituents of this oil are n-Hexadecanoic acid （53. 46%）, （E, E）-9, 12-Octadecadienoic acid （26.96%） and Oleic Acid （9.34%） and 15 chemical components are identified which represents 97. 5% of the total peak. The result tested by agar diffusion method shows that the oil has a moderate antifungal activity against Canidia albicans and no antimicrobial activity to Escherichia coli and Staphylococcus aureus.
出处
《浙江理工大学学报（自然科学版）》
2012年第1期129-132,共4页
Journal of Zhejiang Sci-Tech University(Natural Sciences)
基金
浙江省重中之重学科开放基金项目(SWYX0911)
国家自然科学基金项目(31071729)
关键词
浙贝母
精油
GC/MS
Kovats保留指数
抑菌活性
Fritillaria thunbergii Miq.
essential oil
GC/MS
Kovats retention index
antimicrobial activity
