银鼓鱼病原菌(海豚链球菌)的分离与鉴定

Isolation and identification of pathogenetic Streptococcus iniae from Selenotoca multifasciata

从患病濒死的银鼓鱼(Selenotoca multifasciata)肾脏组织中分离到Yg1菌株,用该菌株对健康银鼓鱼进行人工感染,可使其致病,发病症状与自然病鱼症状一致,再分离菌株的各种特性与原分离菌株相同。通过ATB系统和菌体常规形态特征、生理生化反应指标测定以及16SrRNA测序分析等综合鉴定,Yg1菌株为海豚链球菌(Streptococcus iniae)。药敏试验结果表明,Yg1菌株对恩诺沙星、土霉素、罗红霉素、强力霉素、四环素、左氟沙星等抗生素较为敏感。

One strain Ygl was isolated from the kidney of diseased Selenotoca multifasciata. The healthy Selenotoca rnultifasciata could be infected through artificial infection by these bacteria. The symptoms in artificially infected fish were similar to those infected naturally. The characters of re-isola- ted strains were the same as the initially isolated strain, which was identified as Streptococcus iniae by the means of ATB system,morphological, physiological and biochemical tests, and 16S rRNA sequence analyses. The pathogens were sensitive to the following antibiotics： enrofloxacin, oxytetracycline, rox- ithromycin, doxycycline, tetracycline, levofloxacin.