罗汉果挥发性成分顶空-固相微萃取-气质联用分析

Analysis of the Volatile Compounds from Siraitia Grosvenorii Using Headspace Solid-phase Microextraction GC-MS

本研究采取顶空，固相微萃取．气质联用对罗汉果的挥发性成分进行了定性和定量分析。结果表明，最佳的分析条件为：15mL萃取瓶中放置0．2g样品，在60℃条件下选择DVB／CAR／PDMS（50／30μm）萃取头萃取30min。本方法初步鉴定出35种化合物，占总峰面积的92．3％。经分析，主要的化合物包括2．丁烯酸丁酯（14．43％），2，4．壬二稀醛（15．49％两种），对丙烯基茴香N（5．40％），2-庚醇乙酸（5．00％）和糠醛（4．88％）。

A headspace solid-phase microextraction method coupled with gas chromatography-mass spectrometry （HS- SPME-GC-MS） was used for quality analysis of volatile compounds in Siraitia Grosvenorii. The satisfying SPME conditions were as follows ：DVB/CAR/PDMS （50/30 μm） fiber,0.2 g sample, extraction temperature of 60℃ and extraction time of 30 min. As a restdt,35 compounds which accounting for 92.3% of total peak area were determined and identified. The main components of the volatile compounds were 2-butanoic acid, butyl Easter （14.43%） ;2.4-nonadienal （ 15.49% ） ;benzene,{-methoxy -4-11-prepenyl} （40%） ;2-heptanol acetate （5.00%） and furfural （4.88%）.