HPLC测定黑豆皮提取物中矢车菊素-3—O-葡萄糖苷的含量

Quantitative Determination of Cyanidin-3-O-glucoside in Black Soybean Coat Extract by HPLC

建立高效液相色谱法测定黑豆皮提取物中矢车菊素-3．0．葡萄糖苷的含量。色谱条件：色谱柱为Inertsil ODS-3150×4．6mm 5um；流动相为：10％甲酸溶液（A）．甲醇（B），梯度洗脱程序：0-20minB由5％-60％，20—25rainB60％^-100％，25～30minB100％；流速1．0mL／min；检测波长520nm；柱温30℃；进样量10汕。线性范围为0．01245mg／mL～0．1992mg／mL（r=1），加标平均回收率为96．86％，RSD为1．10％（n=6）。本方法准确度高、精密度高、重复性好、简捷易操作，可以作为黑豆皮提取物中矢车菊素-3-O-葡萄糖苷含量的测定方法。

This article is to establish a HPLC method fdr analyzing content of cyanidin-3-O-glucoside in blgck soybean coat extract. The chromatographic conditions are as follows：column Inertsil ODS-3 150 × 4.6 mm 5 urn;mobile phase A = water ＋ 10% formic acid solution,B = methanol （0 -20 min linear gradient from 5% to 60% B and 20 -25 min linear gradient from 60% to 100% B then 25 - 30 min,isocratic 100% B） ;flow rate 1.0 mL ·min-1 ;detection wavelength 520 nm ;column temperature 30℃ ;injection volume 10 μL. The method is liner within the range of 0. 01245 mg/mL -0. 1992mg/mL （r= 1） ,average recovery is 96.86% ,RSD of 1.10% （n =6）. This method is accurate,precise, repeated and easy to operate. It is equaled for analyzing content of cyanidin-3-O-glucoside in black soybean coat extract.