RP—HPLC法测定山竹果壳中a—Mangostin的含量

Quantitative Determination of a-Mangostin in Fruit Hulls of Garcinia mangostana L. by RP-HPLC

建立高效液相色谱法测定山竹果壳中a—Mangostin的含量。色谱条件：色谱柱为Diamonsil C18（250mm×4．6mm，5μm）；流动相为乙腈-pH3．0磷酸水溶液（70：30）；流速1．0mL／min；检测波长240nm；柱温25℃；进样20山。线性范围内0．02—0．10mg／mL（r=0．9998），加样平均回收率为98．25％，RSD为0．74％（n=6）。本方法准确度高、重现性好，操作快速简捷，可以作为山竹果壳中a—Mangostin含量的检测方法。

HPLC method was established for analyzing the content of a-mangostin in fruit hulls of C, arcinia mangostana L. ,The chromatographic conditions were determined as follows： column Diamonsil C18 （250mm×4.6mm,5μm） ; mobile phase aeetonitrile-pH3.0 phosphate solution （70： 30） ;flow rate 1.0 mL/min ;detection wave-length 240 nm;col- umn temperature 25℃;injection volume 20μL. The method was liner within the range of 0.02 - 0.10 mg/min （ r = 0. 9998 ）, and the average recovery was 98.25% , RSD of 0.74% （ n = 6 ）. This method was accurate, precise, repeated and easy to operate. It could be used for analyzing content of a-mangostin in C-arcinia mangostana L..