何首乌制品中总蒽醌的含量测定

Determination of total anthraquinones in Polygonum multiflorum products

本文以何首乌制品为研究对象，用甲醇、乙醚代替氯仿提取蒽醌，混合碱萃取后，以1，8-二羟基蒽醌为标准品，在525nm波长处用紫外-可见分光光度法进行测定。通过方法学验证，标准品浓度在1．6～8μg／mL范围内线性良好，回归方程为Y=0．0424X-0．0016，R2=0．9998，平均回收率为99．7％（n=5），重复性RSD为1．65％。实验发现，与用药典方法的测得值相比较，偏差为4．17％；制何首乌原料与其制品中的总蒽醌检测，理论值与测得值偏差仅为2．27％。本方法操作简单、安全，稳定性好，为制定含有蒽醌的中药原料、中成药及保健食品的质量标准提供了实验依据。

Based on the research of Polygonum products, the method was determined that the anthraquinone was extracted by methanol,ether and mixture alkali instead of chloroform, and it was detected by UV-visible spectrophotometer at 525 nm with 1,8-dihych：oxy anthraquinone as a standard. The calibration curve indicated that the absorptivity was linearly correlated with the concentration of anthraquinone in the range of 1.6-8μg/mL. The regression equation was Y = 0. 0424X-0. 0016 （R2 = 0.9998）. The average recovery of 1,8-dihydroxy anthraquinone was 99.7% （ n = 5 ）. The re- peatability RSD was 1.65%. The deviation was 4.17% being compared this method to the method of Pharmacopoeia. When the Polygonu, m multiflorum materials and its products were detected at the same time, the deviation of the theoreti- cal value and measured value was only 2.27%. The method was simple,safe and accurate to determine the content of total anthraquinone in samples. It provided a basis for the establishment of the quality standards about the health food,the Chinese medicine and its materials that contain anthraquinone.