人参皂苷Rh2诱导人肝癌HepG2细胞凋亡的研究

Induce apoptosis effect of Ginseng Rh2 on HepG2 cells

目的研究人参皂苷Rh2(G-Rh2)对肝癌HepG2细胞增殖抑制和凋亡诱导的作用,并探讨其作用机制。方法用终浓度为5、10、20 mg/L的G-Rh2作用于HepG2细胞,于24 h、48 h及72 h后采用MTT法检测细胞增殖抑制率;72 h后收集各组肝癌HepG2细胞,流式细胞术检测细胞周期及凋亡率;RT-PCR检测survivin mRNA表达。结果 MTT法检测显示,G-Rh2对肝癌HepG2细胞有增殖抑制作用,并存在浓度和时间依赖关系;流式细胞仪检测显示G-Rh2作用后,细胞被阻滞于G0/G1期,随药物质量浓度的增加,细胞凋亡率逐渐增加。RT-PCR结果显示,G-Rh2下调肝癌HepG2细胞survivin mRNA表达。结论 G-Rh2在体外对肝癌HepG2细胞有明显的增殖抑制和凋亡诱导作用,初步推断G-Rh2诱发肝癌细胞凋亡的作用与其对survivin基因表达的抑制有关。

Objective To investigate the effects of genoside Rh2 on inducing hepatocellular carcinoma cell hepG2 apoptosis in vitro.Methods MFC cells were divided into four gourps：cont rol group and G-Rh2（5,10,20 mg/L） groups.The cell viability was determined by MTT;the cell cycle was detected by flow cytomet ry;the gene expression of survivin was observed by RT-PCR.Results Compared with cont rol group,the cell viabilities decreased gradually with the increasing of dose and time in G-Rh2（5,10,20 mg/L） groups at different time（24,48,72 h）（P0.05）,the ratio of FCM G0/G1 cells increased and S cells decreased（P0.05）,at the same time,the expression level of surviving decreased in a dose2dependent manner（P0.05）.Conclusion G-Rh2 can decease cell viability by inducing apoptosis and arresting cell cycle,and the process may associate with survivin decrease.