危重症手足口病患儿淋巴细胞亚群的检测

Analysis on peripheral blood lymphocyte subsets in children with critical hand-foot-mouse disease

目的研究危重症手足口病患儿外周血淋巴细胞亚群的变化,以探讨其免疫功能改变。方法以临床确诊并入住ICU的40例危重症手足口病患儿为研究对象,并与30例正常儿童进行比对研究,应用流式细胞仪检测外周抗凝全血的淋巴细胞亚群:T淋巴细胞(CD3+CD19-)、辅助T细胞Th(CD3+CD4+)、抑制T细胞Ts(CD3+CD8+)、B细胞(CD3-CD19+)和NK细胞(CD3-CD56+)的相对计数,并计算Th/Ts值。结果危重症手足口病患儿外周血T淋巴细胞、Th细胞、Ts细胞的百分率下降,均明显低于对照组(P<0.05);B细胞的百分率则显著增高(P<0.05);而两组间NK细胞百分率及Th/Ts细胞比值的变化不存在明显差异(P>0.05)。结论危重症手足口病患儿出现细胞免疫及体液免疫功能紊乱。

Objective To study the variation of peripheral blood lymphocyte subsets and immune function in critical hand-foot-mouse disease（HFMD） children.Methods Forty cases with critical HFMD and thirty healthy children as controls were enrolled in the study.The percentage of lymphocyte subsets,including CD3＋CD19-cells（T）,CD3＋CD4＋cells（Th）,CD3＋CD8＋cells（Ts）,CD3-CD19＋cells（B）,CD3-CD56＋cells（NK）,in anticoagulative whole blood of patients from the two groups were determined by flow cytometry.Results Compared with normal controls,the Critical HFMD children showed an obvious decrease in percentage of CD3＋CD19-cells,T helper cells,T suppressor cells,but obvious increase of CD3-CD19＋cells.No significant difference was found in the ratio of Th/Ts and CD3-CD56＋cells between the two groups.Conclusion There are functional disorders in cellular and humoral immunity in critical HFMD children.