重庆地区汉族群体19个STR基因座遗传多态性的研究

Genetic Polymorphisms of Nineteen STR Loci in Han Population of Chongqing

采用GoldeneyeTM20A试剂盒复合扩增180例重庆地区无血缘关系汉族个体的19个STR基因座。扩增产物经ABI3130序列分析仪进行检测,GeneMapper ID V3.2.1基因分析软件进行基因分析,得到基因分型图。总共检测出182个等位基因,其频率分布介于0.0028～0.4972。19个STR基因座的杂合度介于0 6611～0.9111,个体识别率介于0.8114～0.9882,匹配概率介于0.0118～0.1 886,多态信息含量介于0.6309～0.9190,累积个人识别率和累积多态信息含量均大于0.9999999999,累积匹配概率为3.7189×10^(-24),累积非父排除率为0.9999999964。说明该19个STR基因座具有比较高的多态性,是较理想的遗传标记,所得到的等位基因频率等数据可为重庆地区汉族人群的法医学个人识别、亲子鉴定及群体遗传学等研究提供基础数据。

180 specimens of peripheral blood were collected from the unrelated individuals in Han population of Chongqing, The DNA samples were extracted with Chelexl00 method and amplified with GoldeneyeTM20A PCR Amplification kit. The PCR products were analyzed with an automatic genetic analyzer. The relative Fragmen＇S lengths of PCR products were calculated with gene scan analysis software and afterward genotyped with genotype software. Among the Hun population in Chongqing, we observed 182 alleles in 19 STR loci with frequencies of 0. 0028 ~ O. 4972, The heterozygosities of the 19 STR loci were found to be 0. 6611～0. 9111, The power of discrimination （DP） were 0. 8114 ～0. 9882, The population match probability （ PM ） were 0.0118～0. 1886, The polymorphism information content （ PIC ） were 0. 6309～0. 9190, The combined discrimination power and polymorphism information content for the 19 STR loci were both higher than 0. 9999999999, The total probability of matching were 3. 7189 x 10 -24 ,The combined exclusion probability were 0. 9999999964. Our results indicate that the 19 STR loci demonstrate higher polymorphism, they are satisfactory genetic markers. The data on the allele frequencies of these 19 STR loci can be used in individual identification, paternity testing and other population genetic researches for the Chongqing Han nationality.