小鼠皮肤局部缺血模型的建立

Establishment of Mouse Model of Skin Ischemia

目的研究建立稳定的昆明小鼠皮肤局部缺血模型。方法取40只雌性昆明小鼠,随机分为A/B两组,每组20只。分别在小鼠背部建立深达皮下筋膜层的U型皮瓣。A组为3.3×1.5 cm皮瓣组,B组为2.5×1.5 cm皮瓣组。皮瓣游离缘垫以医用橡胶,缝合切口,造成皮肤缺血模型,术后观察皮瓣发绀水肿及坏死情况,并用散斑全景实时血流成像仪(Moor FLPI)检测皮瓣血流。结果肉眼观察,3 3×1.5 cm皮瓣组在术后第2天,50%小鼠皮瓣末端坏死,坏死面积达皮瓣面积的(37.20±4.83)%。术后第3天100%小鼠皮瓣末端坏死。并随时间的延长,坏死面积逐渐扩大。2.5×1.5皮瓣组在术后第1天皮瓣出现发绀,水肿,发绀水肿面积占(77.46±4.51)%,术后第3,第5天发绀水肿区面积逐渐减小,至术后第7天,发绀、水肿完全消退。散斑全景实时血流成像仪(Moor FLPI)检测皮肤血流显示,术后第1天皮瓣显著性缺血(p<0.05),术后第7天血流基本恢复到术前水平。结论成功建立了昆明小鼠皮肤局部缺血模型,为进一步研究组织微血管再生的机理和策略提供简单而稳定的动物模型。

Objective To establish the model of acute skin ischemia in Kun Ming mice. Methods Forty female kun Ming mice were divided into A and B two groups randomly,20 in each group . Group A U-shaped peninsular incision,which is 1.5 cm in width and 3.3 cm in length, as deep as full thickness,was created on the back of A-group kun Ming mice. Group B U-shaped peninsular incision, which is 1.5 cm in width and 2.5 cm in length,as deep as full thickness,was created on the back of the other female kun Ming mice. Before suturing flaps back in place, a 2-mm-wild medical rubber sheet was inserted to separate the flap edges. Then the flaps were observed and the blood flow of flaps were measured by The full-field laser perfusion imager （FLPI; Moor Instruments Ltd）. Results 50% of the longer flaps had approximately （ 37.20 ± 4. 83 ） % necrosis on the second postoperative day . All of the longer flaps developed a considerable amount of distal necrosis rate on the third postoperative day,whlch was increase with time. The smaller flaps displayed cyanosis and edema on the first postoperative day. The area of cyanosis and edema accounted for （77.46 ±4.51 ） % , and was followed by a progressive decrease over the course of 5 d. Cyanosis, edema completely dissipated until the seventh day. There was a significant decrease in the flap blood flow on the first day after operation （ p 〈 0. 05 ）. There was no distinctly significance until the seventh day. Conclusion We have Successfully established a Kun Ming mouse model of skin ischemia,which provided the possibility to investigate the mechanism and strategy of mierovaseular regeneration.