白囊耙齿菌腐朽木材相关cDNA片段的序列克隆与分析

Cloning and Analysis of Decay-related Genes of Irpex lacteus

为了解白囊耙齿菌腐朽木材的分子机制,利用基因差异显示技术(Differential display reverse transcriptase,PCRDDRT-PCR)研究了白囊耙齿菌腐朽白桦木材前后基因表达的差异.共分离出5个腐朽后在菌丝体中特异表达的cDNA片段,分别命名为A2、A7、C6、B8和B3.序列同源性比对发现:片段A2、A7和C6分别与编码漆酶(Laccase)、纤维素酶A(Cellulase A)和麦芽糊精磷酸化酶(Maltodextrin phosphorylase)等3个基因具有高度同源性;B8与一个编码响应调节蛋白的基因具有高度同源性.研究结果显示,所筛选的差异表达基因A2、A7和C6等与木材降解有密切关系.

In order to know molecular mechanism of wood decayed by Irpex lacteus, the expression of the decay-related genes of I. lacteus were analyzed after inoculated with Betula platyphylla Suk wood, by the method of differential display reverse transcriptase PCR （DDRT-PCR）. Five special expressed cDNA segments of the mycelium of I. lacteus were obtained from the decayed wood, which were named as A2, A7, C6, B8 and B3. Among them, A2, A7 and C6 were highly homologous to known genes including laccase, cellulase A and maltodextrin phosphorylase, respectively. B8 was highly homologous to responding regulated protein. This study suggested that A2, A7 and C6 had a close relationship with wood decay. Fig 2, Tab 2, Ref 22