一株烟草内生拮抗放线菌发酵条件优化

Optimization of Fermentation Conditions of an Endophytic Actinomycetes from Tobacco Plant

为提高烟草内生放线菌Y12发酵时产生抑菌物质的产量,通过摇瓶正交优化试验,确定了Y12的三角瓶发酵条件.发酵培养基组成为:黄豆粉1.5%,蛋白胨1.5%,葡萄糖1.5%,可溶性淀粉1.5%,MgSO4.7H2O 0.05%,(NH4)SO40.25%,NaCl 0.4%,KH2PO4 0.1%,CaCO3 0.5%;培养基初始pH 7.5,培养温度30℃,发酵时间72 h.经过优化发酵液的抑菌圈直径增加了44.9%.

In order to increase the yield of the antibacterial substance produced by an endophytic actinomycetes, Y12, from tobacco plant in the process of fermentation, a series of orthogonal experiments on Y12 were carried out in the shaking flasks to obtain optimal components of the fermentation media as well as the fermentation conditions. It was concluded that the optimal proportion of the fermentation medium was： 1.5% soybean flour, 1.5% peptone, 1.5% glucose, 1.5% soluble starch, 0.05% MgSO4·7H2O, 0.25% （NH4）SO4, 0.4% NaCl, 0.1% KH2PO4 and 0.5% CaCO3. The optimal fermentation conditions were： Medium’s initial pH value 7.5, fermentation temperature 30 ℃, and fermentation time 72 hours. It was observed from the experiments that the inhibition diameters of the zymotic fluid increased by 44.9% under the optimized conditions. Fig 2, Tab 2, Ref 17