稻米抗性淀粉含量测定方法的比较分析

Determination and Properties of Starch in Rice

以高抗性淀粉水稻品种功米3号、粳稻日本晴、中籼品种9311为材料,比较其蒸煮前后抗性淀粉含量的变化,以及在不同pH值下水解后的抗性淀粉含量的差异。研究结果表明,蒸煮前后各材料的抗性淀粉含量依次为:未蒸煮处理>蒸煮隔夜处理>蒸煮处理,未蒸煮处理与其他两种处理相比较差异显著,蒸煮隔夜处理与蒸煮处理相比较差异不显著;不同pH值处理各材料的抗性淀粉含量依次为pH5.2>pH6.0>pH6.9,其中pH5.2的RS含量与pH6.9、pH6.0比较呈极显著差异,pH6.9与pH6.0差异不显著;3份材料抗性淀粉含量大小依次为功米3号>9311>日本晴,功米3号抗性淀粉含量明显高于9311和日本晴,而9311略高于日本晴。总之,消化可溶性淀粉缓冲液的pH值为6.0时以及蒸煮处理后所得抗性淀粉含量的变异系数最低,重现性好,重复间差异小。

The high resistant starch rice,Gongmi 3,a typical Japonica rice,Nipponbare,and Indica rice 9311 were selected to study the cooking and pH effect on their starches.The results showed that the resistant starch content decreased in the order of no cookingcooking and overnight-holdingcooking.A significant difference existed between no cooking and the other two.The pH effect on the resistant starch content was pH 5.2 pH 6.0 pH 6.9.A significant difference was shown between pH 5.2 and the others,but no difference was found between pH 6.0 and pH 6.9.Among the three rice varieties,the resistant starch content decreased in the order of Gongmi 39311Nipponbare.When the pH of the starch hydrolysis buffer was 6.0,cooking seemed to induce the minimal change in the resistant starch content of the rice.The results were also reproducible with minor differences between the replicates.